TB-500
A synthetic version of the 43-amino acid Thymosin Beta-4 peptide, commonly studied in musculoskeletal and connective tissue research models. TB-500 refers to a specific fragment of this larger protein, containing the actin-binding domain of primary interest in cell migration and tissue remodeling research.
Free COA included · Free shipment protection · 99%+ purity by HPLC & MS · Lyophilized powder
Certificate of Analysis — Included Free
Every batch independently tested by U.S.-accredited labs. Lot-matched COA confirms identity, purity ≥99% by HPLC, and safety screening.
Research Overview
TB-500 is a synthetic analogue of the actin-binding domain fragment of Thymosin Beta-4 (Tβ4), a highly conserved 43-amino acid protein found at high concentrations in platelets and blood cells. The specific sequence of TB-500 corresponds to the region of Tβ4 responsible for its G-actin sequestration activity, which modulates cytoskeletal dynamics in cell migration, wound healing, and angiogenesis research.
In vitro studies with TB-500 have examined its effects on actin polymerization equilibria, metalloproteinase secretion, cell motility assays, and endothelial tube formation. It has been used as a tool to dissect actin-dependent signaling pathways in wound healing models and to compare the activity of the full-length Tβ4 protein against the shorter functional fragment.
Animal model research has investigated TB-500 in muscle injury, tendon repair, and cardiac tissue models. It is frequently studied in combination with BPC-157, as the two peptides have distinct but potentially complementary mechanisms involving nitric oxide signaling and cytoskeletal reorganization respectively.
All referenced studies are preclinical or in vitro. Not for human use.
Molecular Information
| Full Name / IUPAC | TB-500 |
| CAS Number | 77591-33-4 |
| Molecular Formula | C₂₁₂H₃₅₀N₅₆O₇₈S |
| Molecular Weight | 4,963.4 g/mol |
| Sequence / Structure | Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-NH₂ |
| Appearance | White lyophilized powder |
| Solubility | Highly water soluble |
| Format | Lyophilized (freeze-dried) powder |
| SKU | CL-002 |
Quality & Testing
- Purity: ≥99% by HPLC
- Identity: Confirmed by Mass Spectrometry (LC-MS)
- Heavy Metals: Screened; below detection thresholds
- Endotoxin: Tested per LAL assay
- Lot Tracking: Lot number on every vial matches published COA
- Packaging: Nitrogen-purged, sealed glass vials
Storage & Handling
- Short-term: Room temperature (15–25°C), dry, dark — up to 4 weeks
- Long-term: Refrigerated at 2–8°C — up to 18 months lyophilized
- Extended: −20°C; avoid freeze-thaw cycling
- Store at −20°C. Stable at 4°C for up to 30 days after reconstitution.
This compound is supplied for in vitro research and laboratory use only. It is not for human or veterinary use, is not a drug or dietary supplement, and has not been evaluated or approved by the FDA or any regulatory agency for any therapeutic application. Purchasers must be 18 years of age or older and qualified researchers operating in compliance with all applicable local, state, and federal regulations governing research chemicals.
