5-Amino-1MQ
A small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using SAM, depleting methyl donor pools. Studied in adipocyte biology for effects on methylation capacity, NAD+ precursor availability, and lipid metabolism gene expression. Often co-studied with NAD+ precursors.
Free COA included · Free shipment protection · 99%+ purity by HPLC & MS · Lyophilized powder
Certificate of Analysis — Included Free
Every batch independently tested by U.S.-accredited labs. Lot-matched COA confirms identity, purity ≥99% by HPLC, and safety screening.
Research Overview
5-Amino-1MQ (5-amino-1-methylquinolinium) is a membrane-permeable small molecule inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that catalyzes the methylation of nicotinamide (and other pyridines) using S-adenosylmethionine (SAM) as the methyl donor. NNMT is expressed at high levels in adipose tissue and liver, and its activity affects the availability of SAM and NAD+ precursors for cellular methylation reactions.
In vitro research using adipocyte cell lines (3T3-L1, primary adipocytes) has characterized 5-amino-1MQ's inhibition of NNMT enzymatic activity, its effects on cellular SAM levels, and downstream changes in gene methylation, histone methylation marks, and lipid metabolism gene expression. Metabolomics studies have used it to map the metabolic consequences of NNMT inhibition on the 1-carbon metabolism network.
Mechanistic studies have examined 5-amino-1MQ's effects on adipocyte differentiation, fatty acid synthesis, and expression of markers associated with the thermogenic (beige/brown) fat phenotype. It is frequently co-studied with NAD+ precursors (NMN, NR) to examine the intersection of NNMT inhibition with the NAD+ salvage pathway.
All referenced studies are preclinical or in vitro. Not for human use.
Molecular Information
| Full Name / IUPAC | 5-Amino-1MQ |
| CAS Number | 41220-95-3 |
| Molecular Formula | C₆H₇N₃ |
| Molecular Weight | 121.14 g/mol |
| Sequence / Structure | N/A (small molecule: 5-amino-1-methylquinolinium) |
| Appearance | Yellow crystalline powder |
| Solubility | Soluble in water or DMSO |
| Format | Lyophilized (freeze-dried) powder |
| SKU | CL-031 |
Quality & Testing
- Purity: ≥99% by HPLC
- Identity: Confirmed by Mass Spectrometry (LC-MS)
- Heavy Metals: Screened; below detection thresholds
- Endotoxin: Tested per LAL assay
- Lot Tracking: Lot number on every vial matches published COA
- Packaging: Nitrogen-purged, sealed glass vials
Storage & Handling
- Short-term: Room temperature (15–25°C), dry, dark — up to 4 weeks
- Long-term: Refrigerated at 2–8°C — up to 18 months lyophilized
- Extended: −20°C; avoid freeze-thaw cycling
- Store at −20°C, desiccated. Stable in solution (DMSO or water) for 30 days at 4°C.
This compound is supplied for in vitro research and laboratory use only. It is not for human or veterinary use, is not a drug or dietary supplement, and has not been evaluated or approved by the FDA or any regulatory agency for any therapeutic application. Purchasers must be 18 years of age or older and qualified researchers operating in compliance with all applicable local, state, and federal regulations governing research chemicals.
